FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_sian020_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_sian020_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8423809
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209120.2482487435315782No Hit
AATAATAATAAGAAAATCATCTCTGCCTAAAAATTTAAGTTAAACTCCAT160240.19022273653165686No Hit
TAATAATAATAAGAAAATCATCTCTGCCTAAAAATTTAAGTTAAACTCCA91170.1082289496354915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC953.1404852E-853.4268041
ATGCGCG1153.168364E-431.514465145
CGACTCC1402.7526343E-531.0655482
CGAGACA6000.026.5772145
ATAACAC31400.023.5428648
ATTCAGG46900.019.634311
CGGAAGC6755.456968E-1217.18122145
AAGACCG3403.2568576E-517.054886145
TCCGTCA5253.6578058E-715.186971145
GAGCACC7353.765308E-1014.792503145
CCGTCTA5506.192895E-714.496654145
TCCCTAT13700.014.2834149
CTCCCTA14500.013.995198
AATAAGA231400.013.9061987
TAATAAG274100.013.3794416
TAAGAAA243100.013.0282269
GTCACGC4000.002143130912.684571145
ATGAGCG12100.012.5797415145
ATGACCG1759.905278E-612.425703145
AATTCAG79500.011.6742121
Produced by FastQC (version 0.11.5)