FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_sian020_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_sian020_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11857717
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAG208150.06.96438846
ATATAAG236550.06.34273866
TAATAAT623000.06.3295293
CGTCTTC48750.06.096194145
TGCCGTC42750.05.934464145
CTCGTAT55400.05.75695145
ATAATAA766700.05.4553862
GCCGTCT41500.05.451140-144
TCTCGTA58700.05.4333057145
ATAATAG258200.05.38982156
AATAATA833900.05.2170181
Produced by FastQC (version 0.11.5)