FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_sian007_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_sian007_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387435
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAGGATCTCGTA23350.602681740162866TruSeq Adapter, Index 18 (97% over 37bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA15430.39826035334959414No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT12990.33528204731116185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8220.21216462116225948No Hit
CAACAATAATATAAAAATTAAAAAGTAATAAATATTCTTATATATCCAAA4280.11047014337888937No Hit
AGATGGAGTCTCGCTCTGTTGCCAAGCTGGAGTGCAGTGGCACAATCTCA3940.10169447778337012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA18000.030.2041935
TCGGAAG18000.030.2041934
ATCGGAA18650.029.9288713
GATCGGA18900.029.1494452
AGAGCAC19000.028.6144989
AGATCGG19350.028.4862541
GAAGAGC19350.028.0969227
GGAAGAG21400.025.4053966
AAGAGCA21650.025.112038
TCTCGTA7400.015.67352740-44
ATCTCGT7550.015.55415840-44
CGTCTTC8000.015.04285150-54
CCGTCTT8250.014.76275650-54
GCCGTCT8300.014.67382450-54
TGCCGTC8450.014.413341550-54
GGATCTC8150.014.053287540-44
TCGTATG8950.013.76906345-49
GATCTCG9300.013.7185540-44
CGTATGC9050.013.61691845-49
TATGCCG8850.013.59700745-49
Produced by FastQC (version 0.11.5)