FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_krian027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_krian027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896110
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATATATATATATATATATATATATATATATATA119371.3320909263371685No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT91661.0228654964234303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGATCGATCTCGTAT14520.16203367890102777TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG15550.014.9161753
CGGAAGA16300.014.6745324
ATCGGAA16050.014.0006692
GATCGGA15950.013.6408321
TAATAAT34400.013.4927921
GAGCACA19800.010.6156479
AGAGCAC20900.010.403728
GAAGAGC21200.010.2564966
AATAATA43800.09.9297712
GCGGTGT1200.00390774439.66853980-84
CGTCTTC5500.09.22699950-54
GCCGTCT5700.09.15762350-54
ATAGGTT6450.00707140668.9951941
ATAATAA38950.08.9324723
TATGCCG5700.08.90205345-49
ATGCCGT5750.08.82464345-49
CCGTCTT6050.08.62784450-54
TGCCGTC5600.08.5443950-54
CGTATGC5950.08.52801745-49
GGAAGAG28050.08.2685775
Produced by FastQC (version 0.11.5)