FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_khyl049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_khyl049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643984
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATATATATATATATATATATATATATATATATA57160.12308397272686555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG55100.05.7883633
CGGAAGA56200.05.67488434
GATCGGA56950.05.475881
ATCTCGT21202.4665825E-45.470825145
ATCGGAA57600.05.411463145
Produced by FastQC (version 0.11.5)