FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_ISO_525_Jun28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_ISO_525_Jun28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122252
Sequences flagged as poor quality0
Sequence length151
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTATATATGTTTTATATTCTGTTATATATAACTATTTGAAATTATATA190960.3728047741501199No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122290.23874264678895143No Hit
TAATAATAATATTAGTAACAACACATCTCTTTACCAGCAACACTTGAGCA89670.17505971982635762No Hit
TAATATATATCATAAAAATGATATTTTATGTAAATTATGTTATGAAAGTA64150.1252378836496135No Hit
TAATATATATGTTTTATATTCTGTTATATATAACTATTTGAAATTATATA57280.11182581411457305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC651.0925343E-555.7843061
CGACTCT752.554622E-548.325162
GGTAGCC855.3523916E-542.636517145
AGCCGAA700.00105833641.418327145
TTAGGGG2450.038.4610023
GAGCCGC1050.007720678627.61222145
TAGGGGG6900.025.2114054
ACCGGAC1952.6211652E-422.302177145
GCAGTGC4652.0736479E-1020.263807145
CAGTGCG1106.170376E-714.501625140-144
TATAGGG4652.9767241E-514.0298852
CCGAGAA3250.00801000713.381307145
CAACAGG3850.001607705413.17856145
CCGGACA3007.230401E-412.080347145
AGCGCAT1409.206184E-611.395403105-109
GACGGCC900.00516325411.278447130-134
CGCCGGA1851.4656507E-710.974203140-144
GGACAAC3357.958981E-610.818221145
CCCGCCG3400.003222977310.659129145
CGGGAAC1451.6442192E-410.0011215140-144