FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_ISO_349_Jun28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_ISO_349_Jun28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13037196
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328520.2519867002076213No Hit
TGCCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAAC204310.1567131459862995No Hit
TATATATATAAACATAAATATTTTTATATATAAGATATATAATGTCTATT192140.14737831662575296No Hit
CCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAACCCC191880.1471788872392499No Hit
TAATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACA176540.13541255343557004No Hit
TATATATATTTATGTATCTGAACTCTGTTCAGATATATGATTTTATATAT171450.1315083396767219No Hit
AAAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATC160800.12333940519111625No Hit
CCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAACCC160690.12325503121990342No Hit
TTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAA158980.12194339948559492No Hit
TTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGA156160.11978035767813877No Hit
AGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCAC154380.11841503341669482No Hit
GCCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAACC151820.11645142099574173No Hit
TGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATC151430.11615227691598716No Hit
AAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCA151230.11599886969560018No Hit
AACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGG150350.11532387792589757No Hit
TTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACA149920.1149940524020656No Hit
GAGATCCTGTCTCAAAATATAATAATAATAATGTTGCCCAGGCTGAAATG148940.1142423570221695No Hit
CATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCT148110.1136057170575636No Hit
TAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGAT146410.11230175568427445No Hit
GCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAG145680.11174181932986205No Hit
CAACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAG145630.1117034675247653No Hit
ATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGA145080.11128159766870115No Hit
TTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAAT144050.1104915504837083No Hit
CAAAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAAT143190.10983189943604438No Hit
AGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGAT140700.10792197954222671No Hit
ACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAG139600.10707823983009844No Hit
TCAAAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAA134400.10308965210003747No Hit
AATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCAC134330.10303595957290201No Hit
CTGGGCAACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTG134240.10296692632372789No Hit
GACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACA133900.10270613404907006No Hit
GAGGCTGCAGTGATCTGTGATTGTGCCACTGCATTTCAGCCTGGGCAACA133150.10213085697261896No Hit
ACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGC131540.10089592884850393No Hit
TGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAACCCCCTCGGG130390.10001383733127892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG1500.082.145918
GCTTGCC1002.740002E-643.49235145
TGTTGGG29100.043.3618551
AGCTACA4700.027.761078145
TCGTTGG2756.7939254E-926.359145
GACTACC1650.002605182121.965834145
TTGCCCC56050.021.59745145
ACTCGCA1700.003097926321.31978145
CGCTAGC350.00365845320.716984125-129
GCGGAGT2556.979234E-519.8969368
TATGGGG9850.018.3984873
ATAGGGG6401.8189894E-1218.1225073
GCAGGTT3602.6260004E-618.121813145
AGAAGGC3703.410847E-617.632034145
ACCAACG2250.016.11115825-29
TGGCTCC2700.002336152416.108278145
GATCGTT2900.003761093414.997363145
CCAGTCC17450.014.954391145
TTAGGGG7805.820766E-1114.869753
TTTCGGG3000.00470832614.4989522
Produced by FastQC (version 0.11.5)