FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59702790
Sequences flagged as poor quality0
Sequence length110
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17427282.9190059627029155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG970000.081.370912
GTATCAA1241400.062.9069679
AAGCAGT1328050.060.135621
GGTATCA1515450.051.942078
GCAGTGG1582800.050.818113
CAGTGGT1621450.048.9091454
AGTGGTA1676350.047.637465
GTGGTAT1716500.046.5962946
TGGTATC1981100.040.046967
AGAGTAC1261900.035.3138818-19
ATCAACG1275800.034.90901610-11
TACATGG1259900.034.39389822-23
AGTACAT1319000.033.49493420-21
CATGGGG407750.031.47943124-25
CATGGGA847800.031.39943324-25
CAGAGTA1277600.031.2374818-19
CGCAGAG1340700.030.0080916-17
GTACATG1321250.029.39656322-23
ACATGGG1266550.029.29474824-25
CAACGCA1549900.028.86424812-13
Produced by FastQC (version 0.11.5)