FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_H1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_H1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6977144
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC212860.30508185010944305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA141630.20299136724138128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT125810.1803173332813541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73110.10478499512121292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT722150.097.140131
AGCAGTG804400.087.0282752
GCAGTGG941350.074.200453
CAGTGGT1004300.069.579594
AGTGGTA1021800.068.50235
TGGTATC1049850.066.611977
GTGGTAT1058550.066.0892266
GGTATCA1059850.065.989798
GTATCAA1086900.064.4352659
CAACGCA1085300.036.48535512-13
ATCAACG1091800.036.27519610-11
GCAGAGT1091900.036.08463316-17
AGAGTAC1091300.035.98734318-19
ACGCAGA1109000.035.68791214-15
TACATGG1104000.034.93488322-23
AGTACAT1149050.034.09016820-21
CATGGGG322800.033.1924424-25
TCAACGC1078400.032.5237512-13
CATGGGA730850.032.2734124-25
AACGCAG1100400.031.90041514-15
Produced by FastQC (version 0.11.5)