FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_H1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_H1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8992622
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC268110.2981444121636604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA179960.2001196091640458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT162550.18075929356310094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132020.14680923984128322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT917450.097.056691
AGCAGTG1029250.086.258242
GCAGTGG1207450.073.367833
CAGTGGT1289100.068.710444
AGTGGTA1309950.067.702765
TGGTATC1342000.065.922267
GTGGTAT1353450.065.4726646
GGTATCA1355100.065.337018
GTATCAA1387750.063.9405259
CAACGCA1388500.036.18486812-13
ATCAACG1396250.036.0232810-11
GCAGAGT1403150.035.7050616-17
AGAGTAC1403300.035.66008818-19
ACGCAGA1421100.035.38914-15
TACATGG1412650.034.72664622-23
AGTACAT1475500.033.7850420-21
CATGGGG407750.033.2317324-25
TCAACGC1380050.032.10359212-13
CATGGGA940450.031.92925624-25
AACGCAG1411650.031.45423114-15
Produced by FastQC (version 0.11.5)