FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_H1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_H1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8106082
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC244520.3016500449909093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA166020.2048091790830638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT147150.18153036201706324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111240.13723029202023862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT837850.097.167431
AGCAGTG936000.086.813172
GCAGTGG1094650.074.0791853
CAGTGGT1164650.069.620554
AGTGGTA1187600.068.3687745
TGGTATC1224550.066.152397
GTGGTAT1233650.065.766116
GGTATCA1236200.065.584448
GTATCAA1265450.064.1122069
CAACGCA1264050.036.355712-13
ATCAACG1271600.036.1309210-11
GCAGAGT1277900.035.83970616-17
AGAGTAC1280600.035.7126218-19
ACGCAGA1295450.035.46587414-15
TACATGG1289800.034.80879622-23
AGTACAT1340500.033.9929920-21
CATGGGG375400.033.25808324-25
CATGGGA852800.032.38994224-25
TCAACGC1255550.032.32764412-13
AACGCAG1283800.031.63172114-15
Produced by FastQC (version 0.11.5)