FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_H1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_H1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852794
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC236080.30063185154226635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA158910.20236109593604518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT142080.18092923359507457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT808600.097.153391
AGCAGTG906850.086.4479142
GCAGTGG1058800.073.9518663
CAGTGGT1123100.069.735134
AGTGGTA1143900.068.46435
TGGTATC1182850.066.12227
GTGGTAT1189400.065.810696
GGTATCA1193950.065.557458
GTATCAA1217950.064.328729
CAACGCA1222600.036.3252312-13
ATCAACG1228000.036.22893510-11
AGAGTAC1233650.035.8334118-19
GCAGAGT1235750.035.78383316-17
ACGCAGA1252200.035.46100614-15
TACATGG1248200.034.84346422-23
AGTACAT1295400.034.03138420-21
CATGGGG367650.033.80949824-25
TCAACGC1215350.032.24614312-13
CATGGGA818200.032.18560424-25
AACGCAG1242100.031.57322314-15
Produced by FastQC (version 0.11.5)