FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_G1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_G1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7929518
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC233820.2948729040024879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA161640.20384593363682382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT142570.17979655257734456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114570.1444854529619581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT812450.096.8477861
AGCAGTG907550.086.5588462
GCAGTGG1059450.073.954673
CAGTGGT1130400.069.232524
AGTGGTA1150400.068.131315
TGGTATC1184550.066.166077
GTGGTAT1194650.065.5729146
GGTATCA1198850.065.433628
GTATCAA1221750.064.28769
CAACGCA1225200.036.25456612-13
ATCAACG1230050.036.13681810-11
GCAGAGT1237050.035.76061216-17
AGAGTAC1237050.035.72210318-19
ACGCAGA1253300.035.44664414-15
TACATGG1245600.034.7639422-23
AGTACAT1296200.034.0056320-21
CATGGGG361100.032.910724-25
TCAACGC1216900.032.32074712-13
CATGGGA831350.032.1705324-25
CAGAGTA1242150.031.586118-19
Produced by FastQC (version 0.11.5)