FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_G1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_G1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7732402
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC230200.2977082671076853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA153360.19833423042412954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT138760.1794526461505752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109320.14137909539623006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT798400.096.7009051
AGCAGTG890800.086.38552
GCAGTGG1041650.073.749593
CAGTGGT1106300.069.4547964
AGTGGTA1124450.068.423765
TGGTATC1156100.066.367297
GTGGTAT1166700.065.897326
GGTATCA1169850.065.707648
GTATCAA1192900.064.532679
CAACGCA1192850.036.56299612-13
ATCAACG1200100.036.3686310-11
GCAGAGT1205350.036.03504616-17
AGAGTAC1205700.035.93908718-19
ACGCAGA1222500.035.6786414-15
TACATGG1216100.035.00935722-23
AGTACAT1265450.034.1040720-21
CATGGGG354850.033.9897124-25
TCAACGC1185450.032.4135712-13
CATGGGA803750.032.2056324-25
AACGCAG1212600.031.74359314-15
Produced by FastQC (version 0.11.5)