FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7558254
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC224350.2968278123492542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA153930.20365814644493294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT134800.17834806821787147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78590.1039790406620365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT781600.097.407131
AGCAGTG875450.086.774862
GCAGTGG1025650.073.851923
CAGTGGT1090350.069.459184
AGTGGTA1108300.068.388085
TGGTATC1140350.066.4727557
GTGGTAT1148600.065.9939966
GGTATCA1151450.065.923568
GTATCAA1176550.064.6172269
CAACGCA1178850.036.5445512-13
ATCAACG1182050.036.46800210-11
GCAGAGT1190750.036.0548516-17
AGAGTAC1191900.035.9694218-19
ACGCAGA1208300.035.6342214-15
TACATGG1205850.034.96762522-23
AGTACAT1253350.034.1766220-21
CATGGGG346550.033.84092724-25
TCAACGC1171000.032.51581212-13
CATGGGA799550.032.3138524-25
CAGAGTA1196700.031.75380918-19
Produced by FastQC (version 0.11.5)