FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_G1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_G1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7310904
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC218700.29914221278244113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA148550.20318964658816474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT133250.18226200207252072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76210.104241554806355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT744250.097.21261
AGCAGTG833850.086.681882
GCAGTGG979600.073.6810463
CAGTGGT1040250.069.406864
AGTGGTA1060500.068.1534355
TGGTATC1090800.066.0980157
GTGGTAT1097600.065.778296
GGTATCA1101700.065.502028
GTATCAA1124050.064.256859
CAACGCA1123150.036.32589312-13
ATCAACG1130700.036.1026710-11
GCAGAGT1134400.035.8352116-17
AGAGTAC1136150.035.76072718-19
ACGCAGA1149700.035.52393314-15
TACATGG1148350.034.7390322-23
AGTACAT1194350.033.910820-21
CATGGGG335400.033.1398324-25
TCAACGC1114950.032.36055412-13
CATGGGA758100.032.08787524-25
AACGCAG1143450.031.61602614-15
Produced by FastQC (version 0.11.5)