FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8532900
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC249000.29181169356256376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA168670.1976701941895487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT154110.18060682769046865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91080.10673979538023415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT858900.096.837781
AGCAGTG960250.086.372192
GCAGTGG1130450.073.2568053
CAGTGGT1211450.068.352014
AGTGGTA1229850.067.393585
TGGTATC1264250.065.48187
GGTATCA1274550.065.0401848
GTGGTAT1274200.065.03416
GTATCAA1303750.063.6957449
CAACGCA1305700.036.10546512-13
ATCAACG1315950.035.8488710-11
GCAGAGT1318750.035.6013416-17
AGAGTAC1314300.035.595818-19
ACGCAGA1339100.035.1867414-15
TACATGG1330900.034.61911822-23
AGTACAT1379750.033.81761620-21
CATGGGG389800.033.22281624-25
TCAACGC1299300.031.98502312-13
CATGGGA880450.031.94803424-25
AACGCAG1328400.031.3050314-15
Produced by FastQC (version 0.11.5)