FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600923
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC256500.2982238069100258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA174830.20326888172350804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT154500.17963188369434305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126470.14704235812830785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT892000.097.269971
AGCAGTG997550.086.715782
GCAGTGG1172500.073.642533
CAGTGGT1250450.069.0365454
AGTGGTA1269900.068.074515
TGGTATC1307850.065.9898157
GTGGTAT1315800.065.620426
GGTATCA1321950.065.373628
GTATCAA1347600.064.277159
CAACGCA1351700.036.26691412-13
ATCAACG1354500.036.23248710-11
GCAGAGT1362600.035.81686816-17
AGAGTAC1363100.035.76284818-19
ACGCAGA1378900.035.54826414-15
TACATGG1378800.034.7922-23
AGTACAT1435050.033.898120-21
CATGGGG395000.033.60793324-25
TCAACGC1340600.032.3063612-13
CATGGGA915050.032.17175724-25
AACGCAG1372150.031.614-15
Produced by FastQC (version 0.11.5)