FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8203348
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC245280.2989998717596766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA164790.20088139622992954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT147540.17985339644252565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115140.14035732727661926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT841050.097.2341461
AGCAGTG935700.087.290512
GCAGTGG1091850.074.7348563
CAGTGGT1168100.069.760384
AGTGGTA1183650.068.937825
TGGTATC1218450.066.865917
GTGGTAT1226650.066.4830866
GGTATCA1234200.066.031478
GTATCAA1258900.064.762319
CAACGCA1255600.036.55894512-13
ATCAACG1264700.036.36195810-11
GCAGAGT1271250.035.92706716-17
AGAGTAC1271100.035.84313218-19
ACGCAGA1287150.035.6438214-15
TACATGG1283800.034.83204722-23
AGTACAT1333500.034.0558920-21
CATGGGG381100.033.17160824-25
TCAACGC1249400.032.6698912-13
CATGGGA842300.032.29214524-25
AACGCAG1281050.031.88613714-15
Produced by FastQC (version 0.11.5)