FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_F1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_F1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8605583
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC259590.3016530082854352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA171010.19871983106780794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT156120.18141711026434817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91210.10598933273899049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT881600.097.264851
AGCAGTG984900.086.92982
GCAGTGG1158900.073.624623
CAGTGGT1232800.069.234494
AGTGGTA1253300.068.145895
TGGTATC1292100.065.9666067
GTGGTAT1301700.065.540196
GGTATCA1304600.065.373838
GTATCAA1330300.064.148859
CAACGCA1334150.036.1945712-13
ATCAACG1340200.036.0485810-11
GCAGAGT1349700.035.66703816-17
AGAGTAC1347750.035.63376618-19
ACGCAGA1368200.035.3308314-15
TACATGG1361700.034.6949222-23
AGTACAT1413550.033.85915820-21
CATGGGG395300.033.42489224-25
TCAACGC1326050.032.2237312-13
CATGGGA900350.032.04760424-25
AACGCAG1356600.031.5448914-15
Produced by FastQC (version 0.11.5)