FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8348101
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC247960.29702563493182466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA166420.19935072659039463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT149160.17867536581073945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121670.14574572109273715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT862250.097.178781
AGCAGTG965950.086.615052
GCAGTGG1132900.073.7867663
CAGTGGT1206200.069.223974
AGTGGTA1222000.068.4284065
TGGTATC1257450.066.369777
GTGGTAT1264600.066.0579156
GGTATCA1269850.065.812538
GTATCAA1295250.064.528519
CAACGCA1298250.036.60064312-13
ATCAACG1306500.036.39332210-11
GCAGAGT1308350.036.19388216-17
AGAGTAC1307350.036.1552918-19
ACGCAGA1322800.035.89546214-15
TACATGG1318450.035.1999322-23
AGTACAT1377800.034.19346620-21
CATGGGG381950.033.8513924-25
TCAACGC1289850.032.4850812-13
CATGGGA876600.032.30210524-25
CAGAGTA1314050.031.85545218-19
Produced by FastQC (version 0.11.5)