FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_E1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_E1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9423760
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC279250.29632545820351963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA190110.20173476404322688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT169790.18017224547314448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102050.10829010925575355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT967500.097.0469741
AGCAGTG1081200.086.615392
GCAGTGG1268850.073.690743
CAGTGGT1350950.069.245934
AGTGGTA1377300.068.025585
TGGTATC1412750.066.196687
GTGGTAT1425100.065.754496
GGTATCA1427650.065.557828
GTATCAA1456550.064.4037869
CAACGCA1458150.036.30002212-13
ATCAACG1464250.036.18977710-11
GCAGAGT1477200.035.72971316-17
AGAGTAC1477800.035.7239118-19
ACGCAGA1496400.035.36631814-15
TACATGG1491600.034.7812222-23
AGTACAT1548300.033.9913320-21
CATGGGG432500.033.15468224-25
TCAACGC1448650.032.32752212-13
CATGGGA986800.032.1214924-25
AACGCAG1484050.031.58893614-15
Produced by FastQC (version 0.11.5)