FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7466334
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC222840.2984597260181503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA150840.2020268581609127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT134980.1807848403245823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT771300.097.22611
AGCAGTG860700.086.95632
GCAGTGG1001800.074.500263
CAGTGGT1068150.069.92424
AGTGGTA1088300.068.723525
TGGTATC1119900.066.665467
GTGGTAT1125350.066.399546
GGTATCA1130750.066.050998
GTATCAA1157300.064.592029
CAACGCA1156900.036.54453712-13
ATCAACG1161650.036.40361410-11
GCAGAGT1168850.036.03042616-17
AGAGTAC1170850.035.91324618-19
ACGCAGA1185900.035.67168414-15
TACATGG1179050.035.0033922-23
AGTACAT1228250.034.13599820-21
CATGGGG342600.033.8975724-25
TCAACGC1149050.032.59483712-13
CATGGGA783050.032.09535624-25
AACGCAG1175900.031.89797214-15
Produced by FastQC (version 0.11.5)