FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_514hpf_E1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_514hpf_E1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7294521
Sequences flagged as poor quality0
Sequence length110
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC219740.3012397935381912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA146500.20083566830501962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT131310.1800118198302534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97170.13320956920954782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT748900.097.143881
AGCAGTG833900.087.0551762
GCAGTGG974350.074.4685443
CAGTGGT1037950.069.873074
AGTGGTA1055650.068.773495
TGGTATC1082700.066.897997
GTGGTAT1092050.066.406356
GGTATCA1093050.066.313928
GTATCAA1116400.065.0438469
CAACGCA1118900.036.61833612-13
ATCAACG1126150.036.4012510-11
GCAGAGT1133500.036.0254816-17
AGAGTAC1131950.035.979418-19
ACGCAGA1147850.035.73261614-15
TACATGG1144100.034.89896822-23
AGTACAT1190700.034.0760320-21
CATGGGG334750.034.03458824-25
TCAACGC1112600.032.661512-13
CATGGGA757800.031.98329424-25
AACGCAG1139800.031.94508614-15
Produced by FastQC (version 0.11.5)