FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_16.5hpf_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_16.5hpf_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49468149
Sequences flagged as poor quality0
Sequence length110
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633270.12801570562100473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC564530.114119895612023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2957100.094.859891
AGCAGTG3324700.084.213522
GCAGTGG3858700.072.4255453
CAGTGGT4080850.068.503314
TGGTATC4243300.065.888727
AGTGGTA4256250.065.803395
GGTATCA4260250.065.671958
GTGGTAT4305250.065.00246
GTATCAA4393450.063.812469
CAACGCA4365050.036.03367212-13
GCAGAGT4394650.035.65111516-17
AGAGTAC4399350.035.59094618-19
ATCAACG4544700.034.6827110-11
TACATGG4467650.034.39142622-23
AGTACAT4661750.033.52005820-21
ACGCAGA4763200.033.0359314-15
CATGGGG1390350.032.8179324-25
TCAACGC4408000.031.84282112-13
CATGGGA2910800.031.80575224-25
CAGAGTA4429900.031.59520318-19
Produced by FastQC (version 0.11.5)