FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_16.5hpf_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_16.5hpf_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59696030
Sequences flagged as poor quality0
Sequence length110
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG911070.15261818918276474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC678490.1136574743747616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3563800.095.000071
AGCAGTG4008250.084.2466662
GCAGTGG4638550.072.689233
CAGTGGT4919600.068.5367364
TGGTATC5124250.065.810227
AGTGGTA5135700.065.7220155
GGTATCA5142900.065.593498
GTGGTAT5193900.064.972566
GTATCAA5297400.063.796379
CAACGCA5264200.036.0207712-13
GCAGAGT5296650.035.6490516-17
AGAGTAC5305950.035.57523718-19
ATCAACG5494000.034.59251810-11
TACATGG5383550.034.4143922-23
AGTACAT5616250.033.54677220-21
ACGCAGA5731300.033.1013714-15
CATGGGG1669550.032.93319324-25
TCAACGC5315900.031.7968412-13
CATGGGA3510600.031.76796224-25
CAGAGTA5344250.031.53532618-19
Produced by FastQC (version 0.11.5)