FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_16.5hpf_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_16.5hpf_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61919801
Sequences flagged as poor quality0
Sequence length110
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730060.11790412569316883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC704040.11370191580557566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3707050.094.739381
AGCAGTG4161300.084.1797562
GCAGTGG4823050.072.479063
CAGTGGT5108500.068.459834
TGGTATC5315700.065.788627
AGTGGTA5329800.065.7038965
GGTATCA5334550.065.5923548
GTGGTAT5385150.064.985196
GTATCAA5491950.063.8588689
CAACGCA5461450.036.06535312-13
GCAGAGT5496500.035.6435416-17
AGAGTAC5501200.035.56373618-19
ATCAACG5693350.034.67574310-11
TACATGG5587250.034.37721622-23
AGTACAT5822800.033.54347620-21
ACGCAGA5957900.033.05556514-15
CATGGGG1736400.032.80242524-25
TCAACGC5505400.031.88418412-13
CATGGGA3633700.031.828624-25
CAGAGTA5544000.031.53005418-19
Produced by FastQC (version 0.11.5)