FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n02_dpErk_WE_16.5hpf_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n02_dpErk_WE_16.5hpf_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62719961
Sequences flagged as poor quality0
Sequence length110
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1033430.16476891623067177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC708260.11292417736037816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3734150.094.6644741
AGCAGTG4178800.084.375012
GCAGTGG4862400.072.3866353
CAGTGGT5154050.068.270684
AGTGGTA5366650.065.654725
TGGTATC5359400.065.628097
GGTATCA5385000.065.341358
GTGGTAT5441050.064.716566
GTATCAA5553200.063.4593629
CAACGCA5515100.035.90342312-13
GCAGAGT5549400.035.54034816-17
AGAGTAC5561100.035.417218-19
ATCAACG5756650.034.4593310-11
TACATGG5644700.034.2239622-23
AGTACAT5899150.033.2916720-21
ACGCAGA6017700.032.91670614-15
CATGGGG1757900.032.52018724-25
TCAACGC5561750.031.73492812-13
CATGGGA3672450.031.62576324-25
CAGAGTA5603800.031.42274718-19
Produced by FastQC (version 0.11.5)