FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59702790
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG76100.018.5006628
CCGGACA43700.018.1116921
CCCGAGT52700.017.574159
GCCCGAA28550.017.0422821
CAAAGAG57100.016.7788775
AGCCGGA22750.016.7768348
GTGGGAC27050.016.6716488
GGGACAA40950.016.3172281
GGGACCT24800.016.2457961
GGGCCAT37400.016.1883541
GCCATGG48650.016.110491
CCCGAGG26800.015.8425619
CACTAAG53950.015.6908521
GAAGGGC19800.015.6662916
GAAAAGG21400.015.62583354
AGGAAGG29550.015.5969584
GAGTGGG30600.015.5649886
TCGGGAC30250.015.3931181
ATGGGAG27750.015.3914791
CCCGGAA28150.015.3682981