FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_G1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_G1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7929518
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA14950.016.4860441
CTGGACG13200.016.0043071
AAACGCT15550.015.566891
GCCCGAA11900.015.5335931
ACGCACG13550.015.509711
GAGACCC17000.015.3394221
GGGCTAC13550.015.2661021
TGCGACG12900.015.1823811
GGCACGT12500.014.9640281
AGTAAAC12700.014.8970448
GAAGCCC19150.014.76637651
ATGGGAG12100.014.7312371
AGCCACG16100.014.6933961
TGCGGAG10950.014.667473
CATGACG12150.014.6660828
AACGGGA9500.014.59341
AATGGAA17600.014.5664211
CCGGGTA11650.014.5446871
GCGGAAA10700.014.499231
TCGGGAC11850.014.4849111