FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7558254
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA14100.017.7121431
GGGACCT11000.016.7027571
AATGGAA16400.015.83188151
GCCCGAA9850.015.63711
GAGACCC15500.015.4735221
GGGCTAC11950.015.3749231
AAACGCT13150.015.3105131
CGAAAAG8400.015.059423
GAACGAG9250.014.983683
TGCGACG13250.014.8628311
GAAGCCC15750.014.8087941
AAACCCA13400.014.778561
TGGGCTG12000.014.7607711
AGCCACG15600.014.7396131
CTGGACG9950.014.7059451
ATGGGAG11850.014.6690871
CATGACG10250.014.4872258
GGGATCC13250.014.4476691
AACGGGA9600.014.4398841
GAAAAGA5950.014.4198774
Produced by FastQC (version 0.11.5)