FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8532900
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA18550.016.6089821
GCCCGAA12650.015.8310511
AATGGAA17800.015.8251871
GGGCTAC14150.015.3970531
TGCGACG14700.015.195241
AAACGCT15650.015.116561
GTGGAGA14600.014.9224861
GGGACCT11850.014.8569781
TGCGGAG11050.014.8332313
CCCGAAG13700.014.6980381
GAAGCCC19200.014.6712661
AACCTGA12950.014.614621
TATATCC17600.014.5045461
AGGCTGC15050.014.4762981
TTGACCC15850.014.4398581
CTGGACG13650.014.4294331
GCGGAAA11450.014.4151
AAACGAA15250.014.35861
TAACGAC13650.014.268211
GAGACCC19700.014.2430451
Produced by FastQC (version 0.11.5)