FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600923
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA17450.017.0246871
CTGGACG13200.016.1710641
GAAAAGA5850.015.7943934
GAGACCC15050.015.6454511
GGGACCT12700.015.4215171
AACGGGA10150.015.3933481
GAAGCCC19300.015.2217561
GCCCGAA12550.015.0797991
GGGCTAC12350.015.0567261
GGCACGT15100.014.9378371
AGCCGAG5450.014.9354133
TGCGACG14900.014.6214261
GAACGAG10200.014.5584663
TGCGGAG11050.014.4340353
AAACGCT14650.014.4203041
GACCGTG15500.014.4103711
GGGATCC14750.014.3971361
GACACGC17550.014.3571811
CTGGCAG15650.014.3425591
AGCGCCA17800.014.3409791
Produced by FastQC (version 0.11.5)