FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8203348
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA12650.016.3523921
CCGGACA14900.016.024611
CTGGACG11900.015.9960651
GGGATCC15700.015.6986511
GAGACCC16400.015.5653231
ATGGGAG14450.015.3053131
AATGGAA18650.015.2804071
GGCACGT12650.015.2216421
CTGGCAG14100.015.2170161
AGCCACG15350.015.1964261
TCGAGAA13350.014.9955988
GGGACCT9750.014.8964671
GAAAAGA7700.014.8573074
TGGAACT12950.014.7840531
GAAGCCC18800.014.74881
AGTAAAC13700.014.6124988
CTCGAGG17850.014.6091221
TAACGAC11400.014.5742461
GGGCTAC14050.014.4880311
ACCCCAA7750.014.477584