FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8348101
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA16500.017.4705811
CTGGACG11650.016.0551011
GGGCTAC14400.015.9688561
AAACGCT15000.015.6235011
GAGACCC17450.015.3214851
AACGGGA10400.015.128391
CGGGAGA3500.015.0862034
AAACGAA15650.014.9746021
GAAGCCC16900.014.973771
ATGGGAG12700.014.81434251
AATGGAA16950.014.7997761
TCGAGAA13750.014.7994178
GAACGAG10150.014.7393943
GGGACCT11450.014.7019841
AGCCACG16950.014.6699531
TGCGGAG10600.014.632553
AGTAAAC13050.014.58179958
GGAATGG15700.014.5765151
TGGAACT15500.014.5516481
TGCGACG14350.014.4910521
Produced by FastQC (version 0.11.5)