FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_16.5hpf_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_16.5hpf_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49468149
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA293150.020.1940577
TCCCGAG321050.019.5562658
CCCGAGT214300.019.1406149
CCGGACA139600.018.9394651
CCCTCCC208550.018.5401045
AAAGAGT238800.018.3566236
CAAAGAG250250.018.2067765
TAACGAC153950.017.9387991
GCCCGAA100900.017.8725741
AGCCGGA90600.017.8105188
CACTAAG180600.017.7407911
GCTTTGA240700.017.6675810
ATGTCCC274100.017.6300131
ACCCGAC125600.017.62096410
GCCATGG185800.017.6114271
TACCCGA125800.017.5927189
GTGGGAC117100.017.3681958
CCTCCCG226600.017.3254876
AAAGCCT175900.017.2475436
AGCCTAG146650.017.2362448