FastQCFastQC Report
Tue 13 Aug 2019
HMJY2BGX7_n01_dpErk_WE_16.5hpf_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJY2BGX7_n01_dpErk_WE_16.5hpf_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59696030
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA351200.020.1914067
TCCCGAG384000.019.6158878
CCCGAGT263600.019.1871249
CCGGACA181900.018.878451
CCCTCCC253450.018.8144935
AAAGAGT293150.018.3416236
CAAAGAG307550.018.1409195
AGCCGGA110250.017.9484928
GCCCGAA127100.017.9369871
ACCCGAC150900.017.84489310
TAACGAC182050.017.8293931
GCTTTGA295250.017.80114710
TACCCGA151450.017.7726029
ATGTCCC339350.017.6447681
GCCATGG226100.017.5773121
CACTAAG212850.017.5652771
CCTCCCG276100.017.506136
GTGGGAC141200.017.410788
AGCCTAG168400.017.2243738
GGGCCAT169750.017.2221371
Produced by FastQC (version 0.11.5)