FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42030757
Sequences flagged as poor quality0
Sequence length110
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37402278.898785715422637No Hit
GGCTACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT7958901.893589496853459No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT4199960.9992587095207445No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2199620.5233358038257555No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086320.25845834753820873No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA591050.14062321075968248No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA504020.119916945583445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT2294200.0102.97132
AGGACCA2289450.0102.751351
CTACATG1562700.0100.749383
ACATGTC963100.0100.653245
TACATGT1000000.0100.3293844
CATGTCA982050.098.510126
ATGTCAT985700.097.797847
TACATGA534600.096.925374
TGTCATC998750.096.531818
GACCATC2246350.096.509883
ACATGAC541950.095.6742865
CCATCCA2088700.095.5295265
ATGACAT554650.095.302357
GTCATCA1013500.094.916559
ACATGTT434850.094.8395541
TGACATC559050.094.4741448
CATGTTA442850.093.580912
ATGTTAC403050.093.118253
ATCCAAT291700.092.602677
GTTACCG361150.091.906785
Produced by FastQC (version 0.11.5)