FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n02_dpErk_HTO2_16.5hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n02_dpErk_HTO2_16.5hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42315803
Sequences flagged as poor quality0
Sequence length110
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2467196258.304369173852145No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10183282.406495748172379No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5309851.2548148974036957No Hit
ACATGTTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4584911.0834982854986825No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440691.0494164555969787No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4185630.9891411017297722No Hit
ACATGTTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3652180.8630770872999858No Hit
ACATGTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2027020.4790219861832706No Hit
ACATGTTACCGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2023790.4782586779695519No Hit
ACATGTTAACGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1008410.23830576959629007No Hit
ACATGTTACCGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA643120.15198104594635722No Hit
ACATGTTAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA546720.12919995870100823No Hit
ACATGTTAACGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530960.12547558178205906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA527520.12466264671853208No Hit
ACATGTTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469400.11092782523824492No Hit
ACATGTTAACGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467090.11038192989035325No Hit
ACATGTTAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462490.10929486556121835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCT154.6190823E-4103.9990544
CCAGTCA154.619299E-4103.9978268
ACATGTT14450500.0103.982151
CATGTTA14455150.0103.9025652
TAAGTTC354550.0103.824283
TAACGTC214000.0103.8035667
ATGTTAC13284800.0103.7994843
GTCAACT44600.0103.77631
TTAACGT521450.0103.698796
CATCTTA99650.0103.6358642
CTTGTCA48700.0103.570738
TGTTAAC843450.0103.56754
ACATCTT99850.0103.540761
TGTTACC13018050.0103.5185244
AAGTTCT228150.0103.497644
TCAACTC40850.0103.491962
GTTACCG11861700.0103.470125
GTTACCA1031450.0103.464425
TTACCGC2570450.0103.4254466
TACCGTC3186200.0103.408797
Produced by FastQC (version 0.11.5)