FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n02_dpErk_HTO1_5-14hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n02_dpErk_HTO1_5-14hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161590472
Sequences flagged as poor quality0
Sequence length110
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5900178236.51315654304172No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93030305.75716493977442No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46804202.8964702819854375No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25397511.571720763338076No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21414451.3252297449814987No Hit
AGGACCATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9755310.6037057679984993No Hit
AGGACCATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020680.3107039627930538No Hit
AGGACCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4192360.2594435147141596No Hit
AGGACCATACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4014720.24845029229198612No Hit
AGGACCATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3695470.22869355812018421No Hit
AGGACCATCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3663170.22669467788917655No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAA2951240.1826370059739661No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2698420.16699128151565767No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2594680.16057134853842125No Hit
AGGACCATACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2507870.15519912584944984No Hit
AGGACCATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2325320.1439020488782284No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAA2320560.1436074770547115No Hit
AGGACCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2297050.14215256453982014No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAA2202690.1363131113324553No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC2054770.12715910626215635No Hit
AGGACCATAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920730.11886406272765884No Hit
AGGACCATACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1915740.11855525739166108No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1713610.10604647531446036No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1682000.10409029562089528No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1646220.10187605615756849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT89520500.0103.881542
GACCATA7696350.0103.8807143
ACCATAC5417650.0103.868284
AGGACCA89851800.0103.833841
CCATACA5217050.0103.810225
TGACCAT627600.0103.7685552
GACCATC82613450.0103.765233
ATCCAAT9414200.0103.7499857
ACCATCC79631950.0103.7449344
ACCATCT1956100.0103.730334
TCCAATA9269300.0103.7210858
CCATCCA76814850.0103.7002645
ATCCAAC15680950.0103.674677
ATCCAGC1195450.0103.654367
TCCAGCA1194050.0103.649828
CATCCAA74560500.0103.649766
TCCAACA15423950.0103.6145558
TCCAAGA6281700.0103.595088
GTACCAT217100.0103.569422
TACCATC169100.0103.538363
Produced by FastQC (version 0.11.5)