FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n02_165SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n02_165SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29733494
Sequences flagged as poor quality0
Sequence length110
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT1641127055.19455601147985No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT842968928.350818776965802No Hit
GGCTACATGTGATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT2067890.695474941491908No Hit
GGCTTCATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT1541540.5184523554480345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG935780.3147225146160085No Hit
GCTACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG789800.26562636735527956No Hit
GGCTACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTACTTTT583690.19630723520081428No Hit
GGCTACATGTCATCAGCCATATCAGCAAAAGTGATACGGGTATTATTTTT551790.18557859362239768No Hit
GGCTTAATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT495270.1665697277286013No Hit
GGCTTCATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT490150.1648477639392128No Hit
GGCGACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT475460.15990720767629932No Hit
GGCTACATGGCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT427650.14382769815077906No Hit
GGCTACATGTCATCAACCATATCAGCAAAGTGATACGGGTATTATTTTTG423820.14253958851926382No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTACTTTT404510.13604522899326935No Hit
GGCCACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT369370.12422690720438034No Hit
GGCTACATGTCATCAACCATATCAGCAAAAGTGATACGGGTATTAATTTT309720.10416535641589919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC154.6188143E-4104.000496104
GCTAGAC252.696488E-7104.000312
GCTACGT31900.0104.000312
CATACTT403.6379788E-12103.999095
ACTGACT252.6967064E-7103.998924
TACTGGC351.6189006E-10103.998914
TGACGTG154.619123E-4103.998748
ATGTACT750.0103.998576
GCTTCAT263900.0103.960912
CTTCATG265700.0103.940913
TTCATGT57750.0103.9088754
GGCTTCA264800.0103.8726351
GGCTACA28046050.0103.87011
TTCATGA204350.0103.820794
GGCTTAA100250.0103.698861
GTGATCA321500.0103.6105659
TTTGGTA363650.0103.5286104
TACATGA9415900.0103.519014
CTACGTG32000.0103.512123
ACATGTG346900.0103.489445
Produced by FastQC (version 0.11.5)