FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42030757
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG3386500.8057194877551218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT144900.020.72296510
GTCCGCG145200.020.70289
GAAGGAA144950.020.641362
AGTCCGC153500.020.1279938
GATCGCT71550.019.1695949
AAGTCCG157950.019.087397
CGACGGC53500.019.080152
GATGCAG93850.019.0569884
TTAGATC71800.018.9808146
GTGGTGA93650.018.76837310
GGCAGAG54900.018.753036
GTTAGAT75150.018.6617035
TCGACGG63900.018.6095431
GGAAGTC162750.018.5249945
AGAGATA49550.018.491386
TGAGAGA51900.018.4594524
CCGCGTC37650.018.40425711
AGATCGC78500.018.2430928
CTAGCGG47350.018.21183610
CCGCGTA37850.018.16171311
Produced by FastQC (version 0.11.5)