FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n01_dpErk_HTO2_16.5hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n01_dpErk_HTO2_16.5hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42315803
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAG229850.020.1042543
CGAAACA230200.019.3473823
ACGAAAA207550.019.2964862
AGAACCA234250.018.9330563
GAAAAGA135050.018.8399524
ACGAAAC195750.018.7626822
GAAGAGG90300.018.272684
AGGGAAG94600.018.26693
AGAAAAG194650.018.134063
ACAGAAA358650.017.7000871
GAGAAAT303150.017.6857341
CCCGAAG127100.017.6468351
TTATACA393300.017.59471322
GAAACAA193050.017.4190464
AGAGGGA86800.017.4127276
CGAAGAG91200.017.2955383
AAGAACC237450.016.982462
GAAATGA189500.016.9673791
TGCGTTC130700.016.8747965
GGGAAGA83150.016.8408854
Produced by FastQC (version 0.11.5)