FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n01_dpErk_HTO1_5-14hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n01_dpErk_HTO1_5-14hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161590472
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAAG510300.018.0849043
CGAAAAG407800.017.4948233
AAACGAA825800.017.4578911
AGAACCA525800.017.2672733
GAAAAGA316500.017.0370464
TTATACA1058600.016.80111522
ACAGAAA1095550.016.7418751
CGAAACA544900.016.5247443
CAGAAAA387700.016.2285822
ACGAAAC386200.016.1349642
GCCCGAA191650.016.1342411
CTGGACG285250.016.1270471
GAGAAAT963750.016.0581421
AATGGAA542950.015.9222321
ACGAAAA356250.015.8704262
CCCGGAA204300.015.78134251
GAAAAGC288550.015.7786244
AAATGGA422650.015.3920671
AAACGCT411900.015.3077341
GAAATAG351550.015.2784563
Produced by FastQC (version 0.11.5)