FastQCFastQC Report
Tue 13 Aug 2019
HMJWTBGX7_n01_165SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJWTBGX7_n01_165SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29733494
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGGAA1767850.021.7873272
AAGGAAG1768800.021.7756253
TCCGCGT1769400.021.72370110
GTCCGCG1771450.021.708979
CGAAGGA1787200.021.692131
AGTCCGC1779550.021.6744448
AAGTCCG1780450.021.5936747
GCAGTGG1058150.021.5646297
CGGCAGA587750.021.5622585
CTGATGC1057000.021.553062
GAAGTCC1782950.021.5399516
CGACGGC587000.021.5385252
ACTGATG1064950.021.5298981
GACGGCA587900.021.4606463
GATGCAG1067050.021.422874
GTGGTGA1070250.021.39188610
CCGCGTC405400.021.38650911
GCAGAGA608950.021.3679647
GGCAGAG597800.021.352496
CTAGCGG495550.021.34995310