FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18422534
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280021.2376256165411337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA80100.010.19511520
GGGGGAA55150.09.62108421
GGGTAAA20750.09.25297122
GGGGAAA75150.08.73304122
AAAGGGG43950.08.29569770
AAGGGGG32800.07.695504770
GGGGGTA15100.07.626859720
GGGGAGA39300.07.461451522
GGGGTTA10502.471279E-87.020745353
GTGGGGA24500.06.694844720
AGGGGGG52050.06.465899570
TCGCTAG6300.00759858546.109471332
AAAAGGG68000.05.93018869
GGGGATA14606.579103E-85.738455322
GGGTTGG38900.05.558420717
CGCCGTA9606.076524E-45.474045347
GGTTGGG37100.05.45301618
GGGGAGG49800.05.3939196
GGGGGAT20507.4396667E-105.30592551
AGCGGTT13201.2095747E-55.2840610
Produced by FastQC (version 0.11.5)