FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n02_QG3141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n02_QG3141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294294
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG12151.8189894E-127.777823470
TCGCTCC8106.051222E-56.48142159
TAGGGGG6050.00497370176.3634838
AAGGGGG12358.5448846E-86.234759369
GGGGTGG14255.7807483E-96.14103916
GGGGGGT16204.2200554E-106.050060714
GGGGGAG15203.8198777E-95.987058621
GGGGGTG14651.0642907E-85.97336615
GGGTGGG14757.6939614E-85.695121317
GGTGGGG14202.1464984E-75.669219518
GGGGGGA15452.0261541E-75.4370920
GTCCGAA10956.412374E-45.114031
GCCCTTT8900.0083847575.11223464
GTTTAAT9600.00363127965.10404161