FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18422534
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1924071.0444111542961463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG88900.049.5329315
CGTCTGA91850.048.0564616
CAGTCAC62550.047.4290427
CACACGT93350.047.3214412
GCACACG93350.047.28387511
GAACTCC93450.046.45082521
ACACGTC97700.045.0001213
CCAGTCA71100.044.9251926
CACGTCT100200.044.01699414
CTCCAGT85150.043.0175324
AGCACAC104100.042.8385310
TGAACTC106600.041.50670220
AACTCCA106550.040.97145522
CTGAACT112250.039.56845519
AGAGCAC114700.038.849148
GATCGGA144850.030.6124041
TCGGAAG146350.030.4691433
CGGAAGA149000.030.1553444
ACTCCAG141900.029.28671523
ATCGGAA165400.027.1578352
Produced by FastQC (version 0.11.5)