FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302361
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTAT46490.14077806757044428TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8400.044.58163546
TATGCCG8750.043.59833548
GTATGCC8950.042.2330347
ATGCCGT9250.040.48494349
ACGATAG11200.040.000932
GATAGCC11550.038.78698734
CGATAGC12150.036.87158233
ATAGCCA12550.035.69639235
TCGTATG9900.034.9986745
AGTCACG13500.033.96475628
CACGATA14300.032.0641431
TAGCCAA15550.028.3594836
TGCCGTC13800.026.88303450
GTCACGA17450.026.67722129
CAGTCAC17700.026.3020227
CTCGTAT13100.026.18220944
TCACGAT17450.026.0754830
ACGTCTG18750.025.1990415
CCAGTCA19400.024.8997326
CGTCTGA20350.023.56175616
Produced by FastQC (version 0.11.5)