FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5125715
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTAT74370.14509195302509015TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14150.044.27402548
CGTATGC14850.041.9517646
GTATGCC14900.041.8109947
CGGTGTA15250.041.31200433
TCGTATG16750.037.19305845
ATGCCGT17000.036.0281249
GGTGTAC19750.032.0763634
CACGGTG19900.031.6608631
ACGGTGT20100.031.51966332
TCACGGT20300.030.51852430
AGTCACG21100.029.69319328
CTCGTAT21050.028.26529344
TGCCGTC22100.028.03066650
GTCACGG22600.027.8772829
CTGCTTG24000.025.08365659
ACGTCTG26700.024.51222815
GCCGTCT26000.023.96067851
TACAATC23750.023.57809838
CAGTCAC28250.022.42594327
GTACAAT26300.021.95845637
Produced by FastQC (version 0.11.5)